Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:500-1:5000Immunohistochemistry (Paraffin): <1:500Immunohistochemistry (Frozen): 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:20ul
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide Reconstitute with sterile ddH2O.
Specificity:Recognizes human Lipoprotein, Low Density, Cu2+ oxidized. Shows strong reactivity to fully oxidized modifications of LDL including Cu2+-oxidized LDL, MDA-LDL, HOCL-LDL. No reactivity to other oxidized proteins like MDA-HSA, MDA-HDL, HOCL-HSA, HOCL-HDL (below detection limit). The reaction to native LDL was weak, but clearly detectable (~20%). Minimally oxidized LDL gave a strong binding signal (>80%).