84-1123-08　Interferon alpha 1 (IFNa1) 100ul　I7662-06D

特徴

• Interferons (IFNs) appear early after viral infection locally and systematically to limit spread of viral infection.
• They also affect cell differentiation, growth, surface antigen expression and immunoregulation.
• There are three naturally occurring interferons: alpha, beta and gamma.
• B-lymphocytes are the predominant cellular producers of INF-alpha.
• IFN-alpha is derived from lymphoblastic tissue.
• IFN-alpha comprises a family of related, homologous proteins, each exhibiting a unique activity profile.
• The activities of the different IFN-alpha species on viruses can vary twenty fold or more.
• INF-alpha has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin.
• Recombinant IFN-alphas from both natural and synthetic genes bind to a common cell surface receptor and induce anti-viral activity in a variety of cell lines.
• When binding to discrete cell surface receptors on target cells, IFN-alpha induces rapid changes in Jak/Stat phosphorylation, which intiates the Jak/Stat signaling pathway.
• IFN-alpha signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon).
• All IFN-alpha signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
• IFN-alpha remains the most frequently used IFN for both research and clinical applications.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.