Glypican-4, previously known as K-glypican, is a GPI-linked cell surface heparan sulfate proteoglycan of the glypican gene family, that comprises 6 members at present.
Its function is only partially understood at present.
However, glypican-4 seems to be involved in the control of cell growth and diffe-rentiation, especially in the development of the central nervous system and the kidney.
Furthermore, glypican-4 is expressed by haematopoetic stem cells and bone marrow stromal cells.
Although the antiserum was prepared against a peptide from hu man glypican-4, due to the highly conserved structure and se-quence of glypican-4, antiserum anti-peptide 1 (aa88-101) was used successfully to study brain development in the mouse.
Applications:Suitable for use in ELISA, Western Blotting, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000-1:2000Immunohistochemistry: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：20ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Specificity：Specific for a core protein epitope (aa 509-520) of human glypican- 4.
