Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells.
FGF-b also exerts a potent angiogenic activity in vivo.
Applications:Suitable for use in Immunohistochemistry, Western Blot and RIA.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:1200Immunohistochemistry (frozen): 1:5000Western Blot: 1:10,000-1:30,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ul
Host:rabbit
Source Antibody:bovine
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile ddH2O.
Specificity:Recognizes bovine Fibroblast Growth Factor-2. Species Crossreactivity: human, domestic ruminants, porcine FGF-2. No cross reaction with bovine FGF-1, IGF-1, IGF-2, TNFa and EGF.
Isotype:IgG
Calc Applications Abbrev:IHC RIA WB
Calc Crossreactivity:Bo Hu Po
Immunogen:Recombinant protein corresponding to bovine Fibroblast Growth Factor-2 [FGF-2] (basic FGF)