The EGF receptor (EGFR), a 180kD transmembrane tyrosine kinase, is part of the ErbB family which mediate ligand induced signaling as heterodimers.
Ligand binding results in activation of the intrinsic kinase activity and autophosphorylation on tyrosine residues within and surrounding the kinase domain.
The phospho-tyrosine residues serve as docking sites for adapter proteins that nucleate signaling complexes that result in the activation of the Ras, PI-3 kinase and PLC-g pathways.
EGFR, which transduces the biological effects of EGF and TGF-a, is frequently over-expressed in solid tumors.
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000Western Blot: 1:500-1:1000Immunofluorescence: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Specificity：Recognizes human Epidermal Growth Factor-Receptor
