Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.
Humans have six elastase genes which encode the structurally similar proteins.
The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils.
The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix.
This protein degrades the outer membrane protein A (OmpA) of E.
coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia.
Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN).
This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter.
All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
Applications:Suitable for use in ELISA, Western Blot and RIA.
Other applications not tested.
Recommended Dilutions:ELISA: 1:8000Western Blot: 1:500RIA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：20ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O.
