Applications:Suitable for use in ELISA, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000Western Blot: 1:500Immunofluorescence (Frozen): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography from serum.
Form:Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 10ul/100ul sterile ddH2O.
Specificity:Recognizes mouse type VII collagen (aa757-967). Species Crossreactivity: human. Not tested for reactivity with type VII collagen from other species.
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Glutathione-S-transferase fusion proteins containing sequences of mouse type VII collagen (aa97-200 and 479-578 and 757-967).