Asymmetric dimethylarginine (ADMA) is a naturally occurring chemical found in blood plasma.
It is a metabolic by-product of continual protein modification processes in the cytoplasm of all human cells.
It is closely related to L-arginine, a conditionally-essential amino acid.
ADMA interferes with L-arginine in the production of nitric oxide, a key chemical involved in normal endothelial function and, by extension, cardiovascular health.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Crossreactivity:Using conjugated ADMA-KLH, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:Compound: Cross-reactivity ratio(a) ADMA-BSA: 1 SDMA-BSA: 1:>100NDMA-BSA: 1:>100L-Arginine-BSA: 1:>5000
(a) ADMA-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement BSA=Bovine Serum Albumin
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography from serum.
Form：Supplied as a lyophilized powder from 50mM Tris, pH 7.4. Reconstitute with 100ul sterile ddH2O.
