Aphrodisin is a protein which is secreted in hamster vaginal discharge and acts via the vomeronasal organ of the accessory olfactory system to elicit copulatory behavior in male hamsters.
The complete primary structure of aphrodisin was determined by sequence analysis of intact aphrodisin after unblocking the amino terminus with pyroglutamate aminopeptidase and from peptides generated by trypsin and Lys-C digests.
Alignment of the peptides was obtained from sequence analysis of peptides from cyanogen bromide and hydroxylamine cleavages.
The protein consists of 151 residues of Mr = 17,000.
It has disulfide bonds linking cysteine residues at positions 38 and 42 and at 57 and 149.
N-acetylglucosamine residues are linked to asparagines at positions 41 and 69.
Based on its similarity to the major urinary proteins in rats and mice, aphrodisin is a putative member of the alpha 2u-globulin superfamily of extracellular proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:20ul
Host:rabbit
Source Antibody:hamster
Grade:Serum
Purity:Serum
Form:Supplied as a liquid.
Specificity:Recognizes golden hamster Aphrodisin (aa 107-126).
Isotype:IgG
Calc Applications Abbrev:E WB
Immunogen:Synthetic peptide corresponding to aa107-126, KIFFTNKNMDRAGQETNMIV of golden hamster Aphrodisin.