84-1120-89　Osteonectin (SPARC, Secreted Protein, Acidic, Cysteine-rich, ON, OSN) 100ug　O8063-12

特徴

• SPARC, an acronym for “secreted protein, acidic and rich in cysteine,” is also known as osteonectin.
• It is the founding member of a family of secreted matricellular proteins with similar domain structure.
• The 302 amino acid (aa), 43kD protein contains a 17aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs.
• Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face.
• SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morpho- genesis and remodeling.
• SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation and promotes collagen matrix formation.
• For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF.
• SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
• SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK.
• Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation.
• Applications:Suitable for use in Direct ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.2ug/ml using C2C12 mouse myoblast cell line and mouse placenta tissueFlow Cytometry (Intracellular staining): 0.25ug per 10e6 cells.
• Detected Osteonectin in Balb/3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin
• Immunohistochemistry: 1-15ug/ml using immersion fixed frozen sections of mouse embryo (E15)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• .
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.