Tyrosinase is widely distributed in both animals and plants.
It is a copper containing oxidase involved in the biosynthesis of melanin in mammals and is involved in fruit browning reactions (Thalmann and Lötzbeyer 2002).
In man, mutations in the TYRP1 gene can lead to the development of Albinism, oculocutaneous, 3 (OCA3), a condition characterized by reduced deposition of melanin in the skin, hair and eyes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:500-1:5000.
In a simple ELISA with 1ug of antigen coated/well, the 50% OD was observed at 1:20,000.
Under the same conditions, the end point was observed at >1:400,000.
Alkaline phosphatase conjugated anti-sheep was used for detection.
All incubations at 18ºC using pNPP substrate, with OD measurements at 405nm
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：sheep
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
