IL-4 (Interleukin-4), also known as B-cell stimulatory factor 1 is an 129aa pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and hematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching.
IL-4 signalling through the IL-4 receptor (IL-4R), under the influence of Stat6, induces the differentiation of CD4+ T cells into Th2 helper cells, which themselves then secrete IL-4 along with IL-5, IL-6, IL-10 and IL-13.
Excessive secretion of IL-4 by Th2 helper cells has been linked with allergy and elevated IgE levels.
Applications:Suitable for use in Immunohistochemistry, ELISA, Functional Assays, and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.03-0.05ug/mlImmunohistochemistry: paraffin, requires antigen retrieval using heat treatment prior to staining of paraffin sections using sodium citrate buffer pH 6.0
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile dH2O.
Specificity：Recognizes human IL-4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL-4, expressed in E. coli.
