CYP46A1 converts cholesterol into the biologically active oxysterol and 24(S)-hydroxycholesterol, in the first step of the enzymatic degradation of cholesterol in the brain.
CYP46A1 is also thought to play a role in the metabolism of neurosteroids and drugs that can cross the blood-brain barrier and are targeted to the central nervous system.
P450 enzymes are divided into two groups: steroidogenic and xenobiotic.
The latter group is comprised of three families 1, 2 and 3.
The xenobiotic p450's are involved in most oxidative drug metabolism.
Work in this area is ongoing but studies suggest that all known drug metabolism is mediated by family members: CYP3A, CYP2D6, CYP1A2, CYP2C9/10, CYP2C19 and CYP2E1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:15,000Western Blot: 1:3000, detects a band of ~58kD in human Alzheimer diseased brain lysates
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.09% sodium azide.
Specificity：Recognizes human cholesterol 24-hydroxylase (CYP46A1).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa sequence 254-270 of human cholesterol 24-hydroxylase protein.
