84-1119-69　［取扱停止］RANKL (Receptor Activator of Nuclear Factor kappa B Ligand, Receptor Activator of NFkB Ligand, CD254, hRANKL2, hRANKL-2, Osteoclast Differentiation Factor, ODF, Osteoprotegerin Ligand, OPGL, sOdf, SOFA, TNF-related Activation-induced Cytokine, TRANCE, Tumor Necrosis Factor Ligand Superfamily Member 11, Tumor Necrosis Factor Superfamily Member 11, TNFSF11) (Biotin) 50ug　R1075-10K1

特徴

• Specific for the soluble form of human CD254, otherwise known as sRANKL, a 20kD glycoprotein and member of the Tumor Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.
• CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists.
• Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.
• Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions.
• Studies have also revealed a link between CD254 and apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.15-0.3ug/mlWestern Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C.
• Reconstitute with sterile buffer, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.