CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists.
Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.
Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naïve T cell proliferation and may be involved in the regulation of DC-T cell interactions.
Studies have also revealed a link between CD254 and apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
