CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.
Applications:Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot.
Western Blot: 0.1-0.2ug/mlRequires antigen retrieval using heat treatment prior to staining of paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile ddH2O. For long-term storage, add 0.09% sodium azide to reconstituted product.
Specificity：Specific for the soluble form of human CD154 (sCD40 ligand). Species Crossreactivity: yeast
