Caspase-2 is required for the permeabilisation of the mitochondria and the resulting amplification of caspase activity, in response to cytotoxic stress.
Cytotoxic stress induced proteolytic cleavage of the caspase-2 proenzyme forms an active caspase which cleaves other caspases in a cascade.
This leads to the release of cytochrome c and Smac from mitochondria and the translocation of Bax from the cytoplasm to the mitochondria, leading to apoptosis.
In adults, caspase-2 is expressed in the placenta, lung, kidney, pancreas and at lower levels in the heart, brain, liver and skeletal muscle.
Applications:Suitable for use in Immunohistochemistry (paraffin) and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 10ug/ml Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Caspase-2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 6aa near the amino-terminus of human Caspase-2.
