The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development.
Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumor suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection.
An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependent upon or delayed by autophagy.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BECLIN-1 (N-TERMINAL). Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 17 amino acid peptide located near human Beclin-1 amino-terminus.
