Bcl-2 homologous antagonist/killer (Bak) is an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells.
Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology.
Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells.
Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced.
When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Frozen): 2ug/ml Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography from whole serum.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes the N-terminal of human BAK. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide corresponding to 13aa near the N-terminus of human BAK.
