BSA is synthesized in the liver as preproalbumin, which has an N-terminal peptide removed before it is released by the rough endoplasmic reticulum as proalbumin.
In turn, proalbumin is cleaved in the Golgi vesicles to produce albumin, which is secreted into the blood stream.
BSA is used as a nutrient in cell cultures, as an enzyme stabilizer during DNA digestion, and as a diluent or blocking agent in immunohistochemistry and ELISAs.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:100,000Western Blot: 1:1000-1:2000Immunohistochemistry (Frozen): 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:rabbit
Source Antibody:bovine
Grade:Serum
Purity:Serum
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide