ATG12, a member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy.
This conjugate also associates with innate immune response proteins such as RIG-I and VISA, inhibiting type I interferon production and permitting viral replication in host cells.
ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7.
Research has demonstrated the existence of at least two isoforms of ATG12.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Paraffin): 2.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Autophagy-related protein 12 (ATG12). Detects a band of ~40kD in mouse heart tissue lysate. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a 15aa sequence from near the center of human ATG12.
