84-1118-83　RPA32 (Replication Protein A 32kD Subunit, Replication Factor A Protein 2, Replication Protein A2 (32kD), Replication Protein A2 32kDa, REPA2, RPA2, HSSB, p32, p34, RF-A Protein 2, RPA p32, RP-A p34, RPA34) 100ug　R3400-02K

特徴

• RPA2 (replication protein A 32kD subunit; also RFA2 and RPA p34) is a 32kD DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex.
• In conjunction with 70kD RPA1 and 14kD RPA3, RPA2 participates in DNA replication, recombination and repair.
• Human RPA2 is 270aa in length.
• It contains a Gly/Ser-rich N-terminus (aa1-33), a DNA-binding domain (aa43-171) and a protein-interaction C-terminus (aa187-270).
• Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes.
• There are multiple splice variants.
• Three contain N-terminal extensions: one shows an 88aa insertion after Ser4, another shows a 12aa substitution for aa1-4, and a third shows a four aa insertion after Ser4.
• There is also a deletion of aa93-98, and a potential truncation after Gln175.
• Over aa141-270, human RPA2 shares 83% aa identity with mouse RPA2.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
• Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.