DEC-205 (Dendritic and thymic Epithelial Cell-205; also Ly-75 and CD205) is a 200-205kD member of the mannose receptor family of molecules.
In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium.
DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance.
Mature mouse DEC-205 is a 1696aa type I transmembrane glycoprotein (aa28-1723).
It contains a 1640aa extracellular domain (ECD) (aa28-1667) and a 31aa cytoplasmic tail.
The ECD is highly modular, containing a ricin B-type lectin domain (aa33-182), a FN type II domain (aa164-211) and nine sequential C-type lectin domains (aa225-1662).
There are at least two potential isoform variants.
One shows a two aa substitution for aa866-1723, and another shows a 64aa substitution for aa734-1723.
Over aa216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes mouse DEC-205. Species sequence homology: rat (87%), human (79%).
