PLTP (phospholipid transfer protein) is a 65-81kD, secreted glycoprotein member of the BPI/LBP family of proteins.
It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL.
Mature human PLTP is 476aa in length.
It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain.
There is one essential intrachain disulfide bond (Cys146-Cys185).
There are four potential PLTP splice variants.
Two show alternate start sites at Met21 and Met89.
Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa110-162, and a second shows a four aa substitution for the N-terminal 35aa.
Over aa18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
The detection limit for rhPLTP is ~0.3ng/ml
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4,5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human PLTP.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：NS0-derived Recombinant protein corresponding to aa18-493 with a Glu18Val substitution from human PLTP (P55058) expressed in NS0 cells. Species Sequence Homology: porcine; 92%, mouse; 83%
