MUNC18-2 (Syntaxin-binding protein 2; STXBP2, Unc18-2, Unc18B) is a 64-66kD member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins.
It is widely expressed, particularly in hematopoietic cells, and is found both in cytoplasm, and associated with membranes, where it binds to STX1A, 2 and 3.
Thus, it presumably participates in primary granule exocytosis by promoting granule fusion.
Human STXBP2 is 593aa in length.
It contains one SEC1 domain (aa28-576) with Trp28, Ser42 and Glu59 all playing a key role in syntaxin binding.
There is one splice variant that shows a deletion of aa83-85.
Over aa80-223, human and mouse STXBP2 share 95% amino acid sequence identity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/ml.
The detection limit is ~1ng/well
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human MUNC18-2 (Syntaxin-BP2). Shows ~5% crossreactivity with recombinant human Syntaxin-BP1 and Syntaxin-BP3 in direct ELISA. Species Sequence Homology: Mouse - 95%.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to aa81-223 of human MUNC18-2 expressed in E. coli (NP_001120868).
