84-1118-71　HMGA1B (High mobility group A1, Isoform B, HMG-Y) 100ug　H6205-71

特徴

• HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10-11kD member of the HMGA family of proteins.
• It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue.
• HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences.
• It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well.
• Human HMGA1B is 96aa in length and contains three A-T hook DNA-binding domains (aa21-31; 42-52; 67-78).
• It is constitutively phosphorylated, and may undergo acetylation.
• There are three potential splice variants.
• All three show the same 11aa insertion after Pro34.
• In addition, one form shows an additional 56aa substitution for aa55-96, while a second shows a 14aa substitution for aa80-96.
• Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
• Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.