FUT11 (Fucosyltransferase 11; also FucTXI and alpha 1,3 fucosyltransferase 11) is a 56kD (predicted) member of the glycosyltransferase 10 family of enzymes.
It is potentially an ER associated enzyme that catalyzes the addition of alpha-L-fucose to a biantennary N-glycan acceptor.
Rather than serve as a permanent modification, this fucosylation may serve as a quality control mechanism, flagging inappropriately glycosylated molecules.
Human FUT11 is a type II transmembrane protein 492aa in length.
It contains a short cytoplasmic region (aa1-7) plus an extended lumenal domain (aa25-492).
that possesses a transferase region (aa60-360).
There are two potential splice variants.
One shows an Arg sunstitution for aa445-472, while a second shows a 31aa substitution for aa446-472.
Over aa25-492, human FUT11 shares 88% aa identity with mouse FUT11.
Applications:Suitable for use in ELISA, Immunoprecicpitation, and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity：Recognizes human FUT11. Species sequence homology: mouse (88%).
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：CHO cell-derived recombinant human Fucosyltransferase 11 (aa25-492; Accession # AAH36037).
