FoxO1, also called FKHR (forkhead in rhabdomyosarcoma), is a 655aa, 70kD, ubiquitously expressed member of the forkhead box O family of winged helix transcription factors.
In neurons, it is activated in response to stress, translocating to the nucleus where it promotes apoptosis and blocks proliferation.
In insulin-responsive tissues, nutrient abundance triggers phosphorylation by AKT that blocks nuclear translocation and activity.
A 60kD form, cleaved at R537, has been found in androgen-treated prostate cancer cells.
Over aa383-655, human FoxO1/FKHR shares 93% aa identity with mouse and rat FoxO1/FKHR.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium azide.
Specificity：Recognizes human FoxO1. Species sequence homology: mouse, rat (93%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human FoxO1 (aa353-655; Accession # Q12778).
