84-1118-66　FASN (Fatty Acid Synthase) [KO Validated] 100ug　F0020-13A

特徴

• FASN (Fatty Acid SyNthase; also FAS) is a cytosolic member of the short chain dehydrogenase/ reductase family 27X.
• It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA and likely mediates the transfer of fatty acids to peptides.
• Human FASN is 2511aa in length.
• It contains multiple domains, including a B-ketoacyl synthase domain (aa1-414), an acyl and malonyl transferase domain (aa429-817), an enoyl reductase domain (aa1563-1863), a B-ketoacyl reductase domain (aa1864-2118) and a thioesterase domain (aa2207-2511).
• FASN exists as an antiparallel homodimer.
• There is one theoretical alternative start site at Met2073.
• Over aa10-212, human FASN shares 92% aa identity with mouse FASN.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-1ug/mlWestern Blot: 1ug/mlKnockout: Fatty Acid Synthase/FASN is specifically detected in HEK293 human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293 cell lineOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.