CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30kD, secreted glycoprotein that bears similarity to the C1q/TNFa-related family of proteins.
It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts and smooth muscle cells.
Functionally, it is recognized to be induced by BMP-2, and to block TGFbeta-induced collagen type I and III synthesis.
Mouse CTHRC1 is 245aa in length.
It contains a 32aa signal sequence, a 16aa prosegment (aa33-48), and a 197aa mature region that shows one collagen-like domain (aa59-92).
Proteolytic processing may generate multiple CTHRC1 isoforms.
There is the potential for an intracellular full-length 33kD, 245aa form, plus extracellular isoforms that are 28kD (aa33-245), 26kD (aa49-245), 20kD (aa98-245) and 18kD and 16kD in size, the last two representing variants of the 20kD form with C-terminal processing.
CTHRC1 may undergo dimerization, trimerization and oligomerization.
One mouse splice variant shows a deletion of aa53-126.
Full-length mouse CTHRC1 shares 99% and 93% aa identity with rat and human CTHRC1, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using NIH-3T3 mouse embryonic fibroblast cell lineImmunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of mouse embryo (E13.5)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4,5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse CTHRC1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ser33-Lys245 of mouse CTHRC1, expressed in NS0 cells. Species Sequence Homology: rat; 99%, human; 93%
