CD1c (Cluster of differentiation antigen 1c) is a 43kD member of the CD1 family of molecules.
It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12kD B2-microglobulin.
It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells.
Mature human CD1c is a 316aa type I transmembrane glycoprotein.
It contains a 285aa extracellular domain (ECD) (aa18-302) plus a 10aa cytoplasmic tail.
The ECD shows one Ig-like domain (aa203-296) that associates with B2-microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail.
There are three potential splice variants.
One shows a Trp substitution for aa327-333, a second shows an eight aa substitution for aa298-333, and a third shows a 50aa substitution for aa297-333.
There appears to be no direct mouse counterpart to human CD1c.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1ug/ml.
The detection limit for recombinant human CD1c is ~0.5ng/ml
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CD1c. In direct ELISA shows <1% crossreactivity with recombinant human CD1a, CD1b, CD1d and CD1e.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Purified, NSO-derived, recombinant protein corresponding to aa19-302 (with a Phe300Ser substitution) from human CD1c (Accession #P29017)
