alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40-46kD member of the immunocalin subfamily, lipocalin family of molecules.
In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus).
Circulating AGP-1 and -2 are both 189aa in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool.
In mouse blood, AGP is normally 200-400ug/ml.
In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold.
More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides.
This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching.
AGP is generally considered to be a suppressor of inflammation.
Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/ml.
The detection limit is ~0.3ng/well
Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
