Urokinase Plasminogen Acitivator is a serine protease (SERPIN) that converts plasminogen into the active protease plasmin.
uPA is secreted from cells as an inactive ~55kD precursor which is activated to the two-chain form consisting of an A and B-chain.
The active high MW form is further processed by removal of an amino-terminal fragment to an active low MW form (~35kD).
uPA is a serine protease that activates plasminogen to plasmin.
uPA is involved in the degradation of the vascular basement membrane and extracellular matrix.
By catalyzing plasmin formation, the uPA can generate widespread extracellular proteolysis and thereby play an important role in physiological and pathological processes.
High levels of uPA and plasminogen activator inhibitor type 1 (PAI-1) in breast cancer tissue extracts have been associated with rapid disease progression.
The malignant phenotype of prostatic tumor cells correlates with the expression of both uPA and its cell-membrane receptor (uPAR).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A sepharose chromatography.
Form：Supplied as a liquid in 0.05M sodium phosphate, 0.15M sodium chloride, pH 7.4.
Specificity：Recognizes both free and complexed forms of human uPA.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human uPA.
