84-1118-11　Fibrinogen (Coagulation Factor I) 1ml　F4203-02C

特徴

• Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver.
• The intact protein has a molecular weight of 340kD and is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma.
• Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains.
• Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aa1-16) followed by Fibrinopeptide B (FPB, Bb1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils.
• The chains of fibrin are referred to as a, b and g, due to the removal of FPA and FPB.
• The polymerized fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between g chains and, to a lesser extent, a chains of the fibrin molecules.
• Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aa chain to produce fragment X (intact DE- D, which is still clottable).
• Fragment X is further degraded to nonclottable fragments Y (D-E) and D.
• Fragment Y can be digested into its constituent D and E fragments.
• Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E.
• Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the y chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000Western Blot: 1:10,000Immunohistochemistry: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.