Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism).
Drug half-life and bioavailability may be modulated by selective inhibition of these proteins.
CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Applications:Suitable for use in Immunoprecipitation, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:2500-1:5000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human CES1
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Liver Carboxylesterase-1
