84-1117-83　Glypican 1 (GPC1, FLJ38078) 100ug　G8235-40C

特徴

• Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS).
• This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI).
• Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth.
• In humans, glypican-1 is over expressed in breast and brain cancers (gliomas).
• All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor.
• The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity.
• The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure.
• Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane.
• Glypican functions as coreceptor for a variety of growth factors.
• Glypican-1 has been shown to interact with SLIT2.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2,500–1:3000 Western Blot: 1:800-1:1,500Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.