The L1 (LINE-1), or Long Interspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies.
This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling.
Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition.
ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains.
The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias.
This antibody is intended for use in studying control of L1 retrotransposons.
Applications:Suitable for use in ELISA and Western Blot.
Expect a band at ~149kD using appropriate cell lysate or extract
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906-10: IgY, Chicken (HRP) Rb x Ch I1906-12: IgY, Chicken (FITC) Rb x Ch I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human L1/ORF2.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptides corresponding to regions within the endonuclease domain of human L1/ORF2 (UniProt Accession #O00370) conjugated to KLH. Species Sequence Homology: chimpanzee; 100%, macaque, fruit fly, bovine, canine, opossum, rat; 69-88%
