Vitronectin, also known as serum spreading factor, a 75kD abundant secreted protein found in blood plasma and the extracellular matrix (ECM), involved in cell adhesion, and implicated in signal transduction and cell migration (metastasis) of cancer cells.
Vitronectin acts as a regulator of plasminogen activated proteolysis, by binding via its N-terminal somatomedin B domain to plasminogen activator inhibitor-1 (PAI-1), thereby stabilizing it.
Vitronectin also uses this N-terminal domain to interact with urokinase receptor, another factor in the plasminogen activation system.
In addition, vitronectin contains an RGD (Arginine-Glycine-Aspartic acid) sequence, which acts as a binding site for membrane-bound integrins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.05M sodium phosphate, 0.1M sodium chloride, 1mM EDTA, pH 6.6. No preservative added.
Specificity：Recognizes both native monomeric and polymeric human vitronectin in various tissues and plasma.
