Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA).
This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA.
The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70.
Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE.
Others occur in a variety of autoimmune and mixed connective tissue diseases.
A heterogeneous group of precipitating antibodies called anti-Jo-1 have been found in dermatopolymyuositis.
Anti-Jo is present in ~25% of patients with myositis.
Recently, Jo-1 has been demonstrated to be an RNA-associated 50kD polypeptide antigen.
It has been identified as histidyl-tRNA-synthetase, the RNA being tRNA-his.
The frequency of ANA-positives in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren's syndrome (SS).
Most of these studies are based on tedious fluorescent ANA (FANA).
Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis and immunoblotting are also used to define antibody specificity.
Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases.
Applications:Suitable for use in ELISA and Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：human
Source Antibody：human
Grade：Purified
Purity：Purified by fractional precipitation to obtain the IgG fraction of delipidized and defibrinated donor plasma.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity：Recognizes human Jo-1 Antigen. Shows only the Jo-1 precipitin band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified using CDC-AF refrence serum in immunodiffusion test. Shows no reactivity at a dilution of 1:100 when tested against purified SSA, Sm/RNP, Scl-70, SSB, and Sm antigens.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：N/A. This product is Jo-1 autoantibody derived from single donor human plasma
