C4 consists of three polypeptide chains (alpha, molecular weight 95kD, beta, molecular weight 75kD and gamma, molecular weight 33kD) and is usually present in serum at 200-600mg/L.
It is a protein of the classical complement pathway.
When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a.
C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.
Applications:Suitable for use in ELISA.
Other applications not tested
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Purified by delipidation followed by solid phase absorption and sterile-filtration (Turbidometric grade).
Form：Supplied as a liquid in 0.05M Tris, 0.5M sodium chloride, pH 7.5, 0.05% sodium azide.
