Clostridium botulinum neurotoxins are zinc-dependent proteases that block the release of acetylcholine from peripheral cholinergic nerve endings by targeting specific membrane proteins including synaptosomal-associated proteins (SNAP), vesicle-associated membrane proteins (VAMP), and syntaxins.
Neurotoxin A is characteristically associated with human botulism and known to target and hydrolyse the 197-Gln--Arg-198 bond of SNAP-25.
Applications:Suitable for use in ELISA, Dot Blot and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 20% glycerol. No preservative added.
Specificity：Recognizes C. botulinum Type A neurotoxin complex, neurotoxin or protein isolates.
Isotype：IgG
Calc Applications Abbrev：DB E WB
Immunogen：Type A Neurotoxin and Complexing Proteins Toxoid.
