Lipoprotein(a) [Lp(a)] consists of an LDL-like particle and the specific apolipoprotein(a) [apo(a)], which is covalently bound to the apoB of the LDL like particle.
Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) gene [LPA] located on chromosome 6q26-27.
Apo(a) proteins vary in size due to a size polymorphism [KIV-2 VNTR], which is caused by a variable number of so called kringle IV repeats in the LPA gene.
This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to >50 kringle IV repeats (each of the variable kringle IV consists of 114 amino acids).
These variable apo(a) sizes are known as "apo(a) isoforms".
There is a general inverse correlation between the size of the apo(a) isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.
Apo(a) is expressed by liver cells (hepatocytes), and the assembly of apo(a) and LDL particles seems to take place at the outer hepatocyte surface.
The half-life of Lp(a) in the circulation is about 3 to 4 days.
Applications:Suitable for use in ELISA, Western Blot and conjugation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:200-1:80,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 75mM sodium phosphate, 75mM sodium chloride, 0.5mM EDTA, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human Lp(a) and Apo(a). Does not cross-react with Apo B 48/100. Minor cross-reactivity to plasminogen.
