At least 9 distinct polymorphic forms of apolipoproteins are known.
The apolipoproteins act as stabilizers of the intact lipoprotein particles.
Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease.
In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL).
Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL).
ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:80,000ELISA: 1:200-1:80,000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:goat
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a liquid, 0.09% sodium azide.
Specificity:Recognizes human Apolipoprotein CII (Apo CII).
Isotype:IgG2a
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant human Apo CII, boosted with pure human Apo CII.