Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also ALS8) is a 33kD, ubiquitously expressed, type IV transmembrane protein and member of the VAP family of proteins.
VAP-B may play a role in vesicle trafficking.
It is found in plasma and intracellular vesicle membranes as a homodimer and heterodimer with VAP-A, and interacts with VAMP1 and VAMP2.
Defects in VAP-B are a cause of amyotrophic lateral sclerosis type 8 and spinal muscular atrophy autosomal dominant Finkel type.
Human VAP-B is 243aa in length and contains a 222aa cytoplasmic domain plus a 21aa C-terminal intramembrane segment.
Within the cytoplasmic domain is a major sperm protein (MSP) domain (aa8-111).
A second isoform, named VAP-C, has a 29aa substitution for aa71-99 plus a deletion of residues corresponding to aa 100-243 in VAP-B.
Over aa1-132, human VAP-B is 96% identical to mouse VAP-B.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
Specificity：Recognizes human VAMP B. Species sequence homology: mouse (96%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived, recombinant human VAP-B (aa2-132).
