p130Cas (protein 130kD Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins.
Although its predicted MW is 93kD, it runs anomalously at 105-130kD in SDS-PAGE.
It is found in a wide variety of cell types.
p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31kD fragment promotes anoikis and cell death.
Human p130Cas is 870aa in length.
It contains one SH3 domain (aa3-65), a Pro-rich region (aa74-87), at least 10 phosphotyrosine (aa128-410) and four phosphoserine (aa134-639) sites, an SH3 binding motif (aa635-643) and a HLH dimerization segment that interacts with CASL.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide.
Specificity：Recognizes endogenous human p130Cas at 105-130kD. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：E. coli-derived recombinant human p130Cas (aa66-412).
