NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-46kD member of the homeodomain family of transcription factors.
It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons.
It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype.
NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1).
When overexpressed, it induces b-cell mitosis.
Human NKX6.1 is 367aa in length.
It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337).
Over aa1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.
Applications:Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml using bTC-6 mouse beta cell insulinoma cell line
Immunohistochemistry (Paraffin): 5-15ug/ml detects NKX6.1 in immersion fixed paraffin-embedded human pancreas
Immunohistochemistry (Frozen): 5-15ug/ml detects NKX6.1 in perfusion fixed frozen sections of rat pancreas
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human NKX6.1. Species Crossreactivity: mouse and rat. Shows <1% crossreactivity with recombinant human NKX3.1 in Direct ELISA.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Met1-Pro120 of human NKX6.1 expressed in E. coli
