Mrc2 (C-type Mannose Receptor 2; also MMR2, Endocytic Receptor 180 and uPARAP) is a 180-200kD member of the macrophage mannose receptor family of proteins.
It is widely expressed, being found on fibroblasts, monocytes/macrophages, endothelial cells, keratinocytes and myoepithelial cells.
It binds the carbohydrates Man, Fuc and NAcGlc, type V collagen, and pro-uPA.
Mature human Mrc2 is a 1449aa type I transmembrane protein.
It contains a 1384aa extracellular region and a 44aa cytoplasmic domain.
The extracellular region shows one ricin B-type lectin domain (aa41-167), one fibronectin type II domain (aa182-230) and eight C-type lectin domains (aa244-1393).
Over aa 800-1059, human Mrc2 shares 87% aa identity with mouse Mrc2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.
Specificity：Recognizes human Mrc2. Species sequence homology: mouse (87%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived, recombinant human Mrc2 (aa800-1059).
