84-1116-92　Microphthalmia (Transcription Factor, MITF) 100ug　M3891-04A

特徴

• MITF (Microphthalmia-associated transcription factor) is a member of the MiT/TFE family of molecules.
• Although is has a predicted MW of 58kD, it runs anomalously in SDS-PAGE at 57-66kD.
• MITF-A1 is found in melanocytes and RPEs where it regulates melanin synthesis via tyrosinase and TRP-1 gene expression.
• Human MITF-A1 is 526aa in length.
• It contains a bHLH DNA binding region (aa309-369) and a Leu-zipper domain (aa374-395).
• There are three phosphorylation sites at Ser54/405/414, and two SUMOylation sites at Arg289/423.
• MITF-A1 acts as either a homodimer, or heterodimer with TFE3, TFEB or TFEC.
• Multiple splice variants exist, with each variant expressing one of two isoforms that are defined by the presence (#1), or absence (#2), of aa294-299.
• One variant has a deletion of aa139-194, a second has an 11aa substitution for aa1-118, and three others show variable substitutions over aa1-35.
• Over aa119-289, human MITF-A1 shares 96% aa identity with mouse MITF-A1.
• Applications:Suitable for use in ELISA, Immunohistochemistry, and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlELISA: 0.5-1ug/mlImmunohistochemistry: 3-10ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.