Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs.
Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes.
IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1.
Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.
Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlELISA: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human IL-34.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn21-Pro242 of human IL-34, expressed in NS0 cells.
