Interleukin-4 (IL-4) is a potent lymphoid cell growth factor which stimulates the growth and survivability of certain B cells and T cells.
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells and basophils.
IL-4 elicits many different biological responses, but has two dominant functions.
The first is regulating differentiation of naive CD4+ T cell to the TH2 type.
TH2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled bu TH1 cells.
The second is regulating IgE and IgG1 production by B cells.
Recombinant IL-4 lacks all N-linked oligosaccharides to molecules highly overglycosylated, but all are fully biologically active.
IL-4’s biological activity is species-specific.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/mlELISA: 0.1-0.4ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：goat
Source Antibody：equine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes equine IL-4.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Eq
Immunogen：Recombinant equine interleukin 4 (reqIL-4), derived from E. coli.
